Clossed-vessel assisted microwave extraction of polychlorinated biphenyls in marine mussels
نویسندگان
چکیده
A reliable and simple procedure to extract polychlorinated biphenyls (PCBs) from marine mussels using microwave energy is proposed. Freeze-dried mussels were subjected to MASE (Microwave Assisted Solvent Extraction) in a closed-vessel microwave system with n-pentane-sodium hydroxide (5 %) or dichloromethane-pentane (1:1) at different temperatures (70 and 90 oC), the extraction time was 10 minutes. Sample extract cleanup procedure was performed by using alumina. Gas chromatography-mass spectrometry and gas chromatography-electron capture detector were employed for individual congener identification and determination. The influence of the following variables on microwave extraction of PCBs has been studied: extraction temperature, solvent volume, solvent nature, cleanup procedure time and volume of sodium hydroxide solution. Recoveries for PCBs were between 77 and 106 % with relative standard deviation between 2 to 20 %. Validation of the extraction process was carried out by processing a certified reference material. Microwave Assisted Solvent Extraction was compared to Soxhlet extraction.
منابع مشابه
Microwave-assisted extraction and accelerated solvent extraction with ethyl acetate-cyclohexane before determination of organochlorines in fish tissue by gas chromatography with electron-capture detection.
Focused open-vessel microwave-assisted extraction (FOV-MAE), closed-vessel microwave-assisted extraction (CV-MAE), and accelerated solvent extraction (ASE) were used for extraction before determination of organochlorine compounds (polychlorinated biphenyls, DDT, toxaphene, chlordane, hexachlorobenzene, hexachlorocyclohexanes, and dieldrin) in cod liver and fish fillets. Wet samples were extract...
متن کاملDechlorination of Polychlorinated Biphenyls in Real Waste Transformer Oil Using a Modified Household Microwave Oven in Presence of Sodium Hydroxide and Polyethylene Glycol
This research was carried out to assess the dechlorination and decomposition of Polychlorinated biphenyls (PCBs) in the real waste transformer oil using polyethylene glycol 1000/NaOH through a modified household microwave oven. To do so, the influence of polyethylene glycol (PEG) (1.5-7.5 g) and NaOH (0.3-1.5 g) under microwave (MW) power of 500 W on the dechlorination efficiency of PCBs, exist...
متن کاملValidation of high-throughput measurement system with microwave-assisted extraction, fully automated sample preparation device, and gas chromatography-electron capture detector for determination of polychlorinated biphenyls in whale blubber.
Validation of a high-throughput measurement system with microwave-assisted extraction (MAE), fully automated sample preparation device (SPD), and gas chromatography-electron capture detector (GC-ECD) for the determination of polychlorinated biphenyls (PCBs) in minke whale blubber was performed. PCB congeners accounting for > 95% of the total PCBs burden in blubber were efficiently extracted wit...
متن کاملOptimization of a microwave-assisted extraction method for the analysis of polychlorinated biphenyls in ash samples.
An alternative method for the extraction of polychlorinated biphenyls (PCBs) in ash samples, which is less time and solvent consuming than Soxhlet extraction, is presented. A study was carried out to evaluate the possibilities of microwave-assisted extraction (MAE) to determine exactly which parameters affect the efficiency of the process, since direct extrapolation of extraction conditions for...
متن کاملPatterns of bioaccumulation of polybrominated diphenyl ether and polychlorinated biphenyl congeners in marine mussels.
Marine mussels (Modiolus modiolus) and sediment from 14 stations near a municipal outfall and three reference locations were analyzed for polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) to evaluate and compare patterns of bioaccumulation of individual congeners between these two groups of chemicals. Of the 47 PBDEs and 209 PCBs analyzed, 34 PBDE and 153 PCB congeners...
متن کامل